Archives
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A different example of substrate dependent activation is see
2020-07-15
A different example of substrate-dependent activation is seen in CSN5, a JAMM-type DUB found in the eight subunit COP9 signalosome (CSN) [34]. The CSN deconjugates NEDD8 (neural precursor cell expressed developmentally downregulated 8) from Cullin Ring ubiquitin E3 ligases (CRLs). Through this activ
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br Results br Discussion Repair by NHEJ
2020-07-15
Results Discussion Repair by NHEJ implicitly requires the pairing together of broken chromosome ends. A complex of Ku, XRCC4, DNA ligase IV, and XLF (PEC) is necessary and sufficient for this purpose (Reid et al., 2015). Here, we describe dynamic changes in this complex that are triggered by d
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Midazolam oral clearance is used
2020-07-15
Midazolam oral clearance is used for characterization of both intestinal and hepatic CYP3A activity. However, the identification and subsequent use of metabolic markers of CYP3A activity can eliminate the need for unnecessary administration of probe drugs such as midazolam. Such metabolic markers wo
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SC 79 In this study we observed alterations in mRNA
2020-07-15
In this study, we observed alterations in mRNA levels of CRF receptors in the PFCx and Hip during the development of sensitization. In particular, in the Hip both CRF receptors showed significantly increased SC 79 when the sensitization condition was established. Similarly, CRF2 receptor increase w
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In the course of carbohydrate metabolism
2020-07-15
In the course of carbohydrate metabolism, pyruvate, the end product of glycolysis, is converted to acetyl-CoA, which fuels the citrate cycle for further generation of energy. In the case of elevated levels of acetyl-CoA, acetyl-CoA is converted into malonyl-CoA by the action of acetyl-CoA carboxylas
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br Conclusions Mulan is a
2020-07-14
Conclusions Mulan is a mitochondrial E3 ubiquitin ligase with diverse roles in cell growth, cell death, and mitophagy. To better understand the mechanism of Mulan\'s function we used a modified yeast two-hybrid system to isolate proteins that interact with its cytoplasmic RING domain (amino acids
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The findings of Qiu et
2020-07-14
The findings of Qiu et al. (2016) represent the first instance of a Ub-specific mono-ADP-ribosyltransferase, as well as the first documentation of E1/E2 independent ubiquitination. Post-translational modification of Ub certainly adds layers of complexity to our understanding of the Ub signal and sho
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In general cellular senescence is considered a programmed
2020-07-14
In general, cellular senescence is considered a programmed response to stress that can be activated by oxidative stress, irradiation or action of substances or drugs. These stressors cause DNA damage and, through the action of specific proteins (p53 and p21), lead to cell senescence (di Fagagna, 200
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The murine subfamily members including and have been identif
2020-07-14
The murine subfamily members including , , , and have been identified and their encoded proteins showed deubiquitinating enzymatic activity , , , . It has been demonstrated that is an immediate-early gene induced by IL-3 in B-lymphocytes and is an IL-2 specific immediate-early gene in T-lymphoc
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histamine receptor antagonist Materials and methods Cell cul
2020-07-14
Materials and methods Cell culture and receptor gene transfection. PC12 histamine receptor antagonist were purchased from the Institute of Cell Biology, Chinese Academy of Sciences, Shanghai, China. The cDNA for mouse CysLT1 or CysLT2 receptor (mCysLT1 and mCysLT2, subcloned into pcDNA3.0) was kind
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In vivo study showed that the
2020-07-14
In vivo study showed that the CYP3A activity was increased, when morroniside (10, 30 and 90 mg/kg, i.g.) was daily for seven consecutive days. Further, RT-PCR analysis showed that the induction of mRNA Calcipotriol by morroniside (10, 30 and 90 mg/kg, i.g.) was 1.13-, 1.43- and 1.79-fold higher, re
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For in vitro expression analysis of CXCL
2020-07-14
For in vitro expression analysis of CXCL8 and CXCRs by stimulated leucocytes, peripheral blood leucocytes (PBLs) were isolated using Percoll density gradient centrifugation as described earlier . Cell suspensions were adjusted to 5×10/ml. Four milliliters of tissue culture medium were plated per wel
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br Materials and methods br
2020-07-14
Materials and methods Results Discussion In our tissue array, we demonstrated that 14-3-3 tau mRNA and protein expression levels decreased in the preeclamptic placentas. 14-3-3 proteins are considered to be important in the functional regulation of trophoblasts and maybe associated with adv
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Pahlavanzadeh and Fakouri Baygi employed PC SAFT EoS to pred
2020-07-14
Pahlavanzadeh and Fakouri-Baygi [6] employed PC-SAFT EoS to predict the Licarbazepine of carbon dioxide by aqueous MEA solutions. They used the ideal form of Smith–Missen algorithm to calculate liquid phase concentrations. The results illustrate acceptable accuracies so that the AAD was achieved equ
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We recently employed a functional drug screening
2020-07-14
We recently employed a functional drug screening approach to identify mithramycin as an inhibitor of EWS–FLI1. In our study, we employed a stepwise approach involving a luciferase primary screen and a novel multiplex PCR screen to evaluate over 50,000 compounds to identify mithramycin as an inhibito